Octet RED384 Biolayer Interferometry (BLI) Label-Free Detection System for Biomolecular Interaction Analysis

Overview

Octet systems enable real‑time, label‑free analysis for determination of binding affinity, kinetic parameters, and antibody or protein quantitation. Based on Bio‑Layer Interferometry (BLI), this fluidics‑free instrument platform offers significant advantages over traditional label‑free techniques, including high‑throughput operation in a 384‑well format.

Description

The 16‑channel high‑throughput Octet RED384 instrument provides label‑free molecular interaction analysis using a state‑of‑the‑art, high‑throughput platform that supports rapid characterization and development of synthetic and biological molecules.

The high detection sensitivity and throughput of the Octet RED384 system make it particularly well suited for small‑molecule library screening and analysis of large cross‑competition binding matrices. Target ligands can be batch‑immobilized onto biosensors and returned to the biosensor tray for reuse, extending biosensor lifetime, preserving valuable ligand material, and maximizing experimental flexibility.

Applications

Characterization of the binding affinity and kinetics of purified glycans or glycoproteins with receptor proteins or other glycans.  Quantitation of  IC₅₀ and K_i values relevant to inhibitor design and development.

Associated Equipment:
8‑channel Octet RED96 system

Instrument Status:
Available

User Access:
The Octet RED384 instrument is a GlycoMIP‑supported resource and is freely available to approved users of the GlycoMIP user facility.

For access to this equipment and other GlycoMIP services, please visit the User Portal.

Instrument Demonstration Video

The following video demonstrates operation of the Octet RED384 BLI system. If the video does not play in your browser, a downloadable version is provided.

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Download the Octet RED384 BLI demonstration video (MP4) .