Overview
Nicoya’s OpenSPR is a high‑quality, benchtop surface plasmon resonance (SPR) system
that provides label‑free measurements of binding kinetics, affinity, and
thermodynamic parameters for a wide variety of molecular interactions. The OpenSPR
uses nanotechnology‑based sensors to deliver highly sensitive and reproducible
kinetic data.
Description
The OpenSPR features integrated control and data‑analysis software, enabling remote connection and instrument operation. Microfluidic flow control allows accurate measurement and analysis of molecular interactions while maintaining low sample consumption and high analytical precision.
Binding analyses are performed using injection volumes as low as 150 µL, with sensitivity reaching picomolar affinity ranges.
Applications
The OpenSPR supports characterization and screening of kinetics and affinity, competition assays, concentration analysis, thermodynamic measurements, and epitope mapping or binning for a wide range of biomolecules, including:
- Glycopolymers and glycoconjugates
- Nanoparticles and glycolipids
- Polysaccharides
- Antibodies and proteins
- Cells, nucleic acids, and viruses
Available Sensors
The following surface functionalizations are available for OpenSPR binding studies:
- Carboxyl (amine coupling using EDC/NHS chemistry)
- NTA (histidine‑tagged targets)
- Streptavidin (biotin‑tagged targets)
- Biotin (streptavidin‑coupled targets)
- Protein A (IgG‑based antibodies)
- Thiol (thiol‑ or maleimide‑tagged targets)
Instrument Status:
Available
User Access:
The Nicoya OpenSPR is a GlycoMIP‑supported resource and is freely available to approved users of the GlycoMIP user facility.
For access to this equipment and other GlycoMIP services, please visit the User Portal.