Overview
BioTools’ ChiralRAMAN‑2X ROA spectrometer provides Raman and Raman Optical Activity (ROA) spectra for liquid and gel materials. The instrument utilizes a 2‑watt laser to capture the full vibrational spectrum from approximately 100 to 2000 cm‑1, collecting Raman spectra in as little as 150 milliseconds.
structural characterization.
Description
The ChiralRAMAN‑2X ROA spectrometer measures differences in the intensity
of Raman scattering from chiral molecules when illuminated with left‑ and
right‑circularly polarized light. These differences provide detailed structural
information about molecular chirality.
Although ROA shares similarities with vibrational circular dichroism (VCD), there are so many vibrational bands in an ROA spectrum that it is not hindered by the spectral bands resulting from the use of a solvent. As such, ROA is often used to probe a material’s structure and behavior in aqueous solutions.
Applications
The ChiralRAMAN‑2X ROA spectrometer is used to obtain configurational and conformational information for a wide range of molecules, including:
- Polymers
- Carbohydrates
- Nucleic acids
- Proteins
- Peptides
- Viruses
- Chiral drugs
ROA measurements enable determination of absolute molecular configuration without crystallization and allow the quantification of enantiomeric excess without the need to separate isomers.
Instrument Status:
Available
User Access:
The ChiralRAMAN‑2X ROA is a GlycoMIP‑supported resource and is freely available to approved users of the GlycoMIP user facility.
For access to this equipment and other GlycoMIP services, please visit the User Portal.
Instrument Demonstration Video
The following video demonstrates operation of the BioTools ChiralRAMAN‑2X ROA spectrometer. If the video does not play in your browser, a downloadable version is provided.
If your browser does not support embedded video: Download the ChiralRAMAN‑2X ROA demonstration video (MP4).