Overview
The Bruker Avance III 600 MHz nuclear magnetic resonance (NMR) spectrometer, equipped with a Prodigy CryoProbe, provides significantly enhanced sensitivity for both routine and biomolecular NMR applications.
Description
Bruker Avance III 600 MHz NMR spectrometer with Prodigy triple‑resonance TCI cryoprobe
CryoProbe Prodigy is a new, revolutionary Bruker CryoProbe product that delivers tremendous boosts in sensitivity. Prodigy probes use nitrogen-cooled RF coils and preamplifiers to deliver a sensitivity enhancement over room temperature (RT). As the Prodigy package comprises, in addition to the probe, just a control unit and a liquid nitrogen vessel, it does not require any additional infrastructure, has long service intervals and keeps operating and maintenance costs at a minimum.
Our CryoProbe Prodigy consists of a triple resonance TCI probe at 600 MHz. Based on the same principles as Helium cooled probes but operated at the temperature of liquid nitrogen, the Prodigy probe delivers a sensitivity enhancement over room temperature (RT) probes of a factor of 2 to 3 both for 1H and X-nuclei. Combined with the automatic tuning accessory (ATMA), and our new Sample Case automated sample changer, the Prodigy has the potential to become the probe of choice for our researchers focused not only on small molecule but also on bio-NMR applications.
Applications
Study of the physical, chemical, and biological properties of glycans and glycoproteins and other biological molecules.
Associated Equipment:
Bruker 5 mm 1H‑optimized triple‑resonance TCI Prodigy CryoProbe designed for 1H observation with 13C/15N decoupling and 13C observation with 1H decoupling. The probe includes cooled preamplifiers for 1H (including 19F), 13C, and 2H nuclei. The 1H circuit is tunable to 19F.
Instrument Status:
Available as of April 2021.
User Access:
The Avance III 600Mhz instrument is available to GlycoMIP users with training by the NMR facility staff. Fees associated with recovery of cryogens and other overhead will apply.
Collaborating Laboratories:
This instrument is maintained in part with resources provided by the
NMR Spectroscopy Facility (opens in a new tab)
of the Virginia Tech Department of Chemistry.