2023 GlycoMIP Summer School @ UGA Agenda

DAY ONE – Monday, Sept. 11:
“Mass Spectrometry and Biolayer Interferometry of Glycans – Day 1”

Day One Agenda
Time	Session
8:15 AM	Arrival, Registration, Distribution of Parking Passes
8:30 AM	Welcome, Introductions (Parastoo Azadi)
8:40 AM	Introduction to GlycoMIP (Parastoo Azadi) What is GlycoMIP and how it can help your research Resources available at GlycoMIP for glycomaterial characterization How to interact with GlycoMIP and find what you need GlycoBasics I/II (pre-work videos) Quiz
9:05 AM	Safety Overview (Mark Baine)
9:15 AM	Break
9:35 AM	Mass Spectrometry (MS) Lecture (Instructor: Parastoo Azadi) Introduction to MS Comparison of different MS instruments and capabilities Methods for glycomaterial MS analysis
10:35 AM	Break
10:50 AM	Bio-Layer Interferometry (BLI) Lecture (Instructor: Rob Woods) Introduction to BLI Comparison of BLI with SPR and ITC Assay Considerations
11:50 AM	Break into Groups A & B
12:00 PM	Lunch; GlycoMIP Scholar Presentation – Structural Characterization of Glycomaterials using Mass Spectrometry (Ambrish Kumar)
1:15 PM	Group A: MS Laboratory (Instructor: Stephanie Archer‑Hartmann) Spotting and running MALDI of glycomaterials ESI-MS of glycomaterials (intact and digested) Sequencing and linkage analysis by MS/MS (data interpretation) Group B: BLI Laboratory (Instructors: Paige LaMore, Swanson Mahmoud) Receptor Immobilization Ligand binding and affinity quantification Data Analysis
5:30 PM	Adjourn, Group Dinner (Location TBD)

Day Two Agenda
Time	Session
8:45 AM	Arrival, Parking Passes
9:00 AM	Modeling Lecture Part 1 (Instructor: Rob Woods) Introduction to Molecular Modeling Force fields Energy minimization Molecular dynamics simulation
10:30 AM	Break
10:45 AM	Modeling Lecture Part 2 (Instructor: Rob Woods) Automated ligand docking Positive controls Literature examples
12:00 PM	Lunch; GlycoMIP Scholar Presentation – Biolayer Interferometry of Glycomaterials (Paige LaMore)
1:15 PM	Group A: BLI Laboratory (Instructors: Paige LaMore, Sawsan Mahmoud) Receptor Immobilization Ligand binding and affinity quantification Data Analysis Group B: MS Laboratory (Instructors: Bhoj Kumar, Stephanie Archer‑Hartmann) Spotting and running MALDI of glycomaterials ESI-MS of glycomaterials (intact and digested) Sequencing and linkage analysis by MS/MS (data interpretation)
5:30 PM	Adjourn

Day Three Agenda
Time	Session
8:45 AM	Arrival, Parking Passes
9:00 AM	NMR (Nuclear Magnetic Resonance) Lecture (Instructor: Christian Heiss) Introduction to NMR Special considerations for NMR of glycomaterials Interpretation of carbohydrate 1D and 2D-NMR spectra
12:00 PM	Group Photo – CCRC Outside Patio Lunch Distribute Evaluations
1:15 PM	Pre-Selected Hands-on Experience – Modeling OR NMR Modeling Laboratory (Instructor: Rob Woods – please install VMD & bring laptop) Modeling glycomaterials with GLYCAM‑Web Visualization using VMD Analysis with Chimera NMR Laboratory (Instructors: Jiri Vlach, Christian Heiss) Sample preparation Introduction to NMR instrument 1D and 2D NMR data acquisition and interpretation
3:30 PM	Reconvene Complete, Submit Evaluations Closing Comments (Rob Woods, Parastoo Azadi)
3:45 PM	Adjourn, Afternoon Social Activity (TBD)