2023 GlycoMIP Summer School @ UGA Agenda

DAY ONE – Monday, September 11: “Mass Spectrometry and Biolayer Interferometry of Glycans – Day 1”

8:15 a.m.Arrival, Registration, Distribution of Parking Passes
8:30 a.m.Welcome, Introductions (Parastoo Azadi)
8:40 a.m.Introduction to GlycoMIP (Parastoo Azadi)

  • What is GlycoMIP and how it can help your research 
  • Resources available at GlycoMIP for glycomaterial characterization 
  • How to interact with GlycoMIP and find what you need

GlycoBasics I/II (pre-work videos) Quiz 

9:05 a.m.Safety Overview (Mark Baine)

Break into Groups (A, B)

9:15 a.m.Break
9:30 a.m.Group A:  Mass Spectrometry (MS) Lecture (Instructor: Parastoo Azadi)

  • Introduction to MS
  • Comparison of different MS instruments and capabilities 
  • Methods for glycomaterial MS analysis 

Group B:  Bio-Layer Interferometry (BLI) Lecture (Instructors: Rob Woods/ Paige LaMore)

  • Introduction to BLI
  • Comparison of BLI with SPR and ITC
  • Assay Considerations
10:35 a.m.Break; Groups A and B switch lectures
10:50 a.m.Group A:  Bio-Layer Interferometry (BLI) Lecture (Instructor: Rob Woods/ Paige LaMore)

Group B:  Mass Spectrometry (MS) Lecture (Instructor: Parastoo Azadi)

12:15 p.m.Lunch; GlycoMIP Scholar Presentation – Structural Characterization of Glycomaterials using Mass Spectrometry (Lauren Pepi)
1:30 p.m.Group A:  MS Laboratory (Instructors: Lauren Pepi, Stephanie Archer-Hartmann)

  • Spotting and running MALDI of glycomaterials
  • ESI-MS of glycomaterials (intact and digested)
  • Sequencing and linkage analysis by MS/MS (data interpretation) 

Group B:  BLI Laboratory (Instructors: Paige LaMore, Swanson Mahmoud)

  • Receptor Immobilization
  • Ligand binding and affinity quantification
  • Data Analysis
5:30 p.m.Adjourn, Group Dinner (Location TBD)

 

DAY TWO – Tuesday, September 12: “Mass Spectrometry and Biolayer Interferometry of Glycans – Day 2; Modeling”

8:45 a.m.Arrival, Parking Passes
9:00 a.m.

12:00 p.m.

Modeling Lecture (Instructor: Rob Woods)

  • Introduction to Molecular Modeling and molecular dynamics simulation
  • Modeling the molecular scale
  • Sampling, relaxation, convergence, analysis and visualization

GlycoMIP Scholar Presentation (Instructor: Paige LaMore)

12:00 p.m.Lunch; GlycoMIP Scholar Presentation – Biolayer Interferometry of Glycomaterials (Paige LaMore)
1:30 p.m.Group A:  BLI Laboratory (Instructors: Paige LaMore, Swanson Mahmoud)

    • Receptor Immobilization
    • Ligand binding and affinity quantification
  • Data Analysis 

Group B:  MS Laboratory (Instructors: Lauren Pepi, Stephanie Archer-Hartmann)

  • Spotting and running MALDI of glycomaterials
  • ESI-MS of glycomaterials (intact and digested)
  • Sequencing and linkage analysis by MS/MS (data interpretation) 

Group B:  MS Laboratory (Instructors: Lauren Pepi, Stephanie Archer-Hartmann)

  • Spotting and running MALDI of glycomaterials
  • ESI-MS of glycomaterials (intact and digested)
  • Sequencing and linkage analysis by MS/MS (data interpretation) 
5:30 p.m.Adjourn

 

DAY THREE – Wednesday, September 13: “Modeling and Nuclear Magnetic Resonance of Glycans”

8:45 a.m.Arrival, Parking Passes
9:00 a.m.NMR (Nuclear Magnetic Resonance) Lecture (Instructor: Christian Heiss)

  • Introduction to NMR
  • Special considerations for NMR of glycomaterials
  • Interpretation of carbohydrate 1D and 2D -NMR spectra
12:00 a.m.Group Photo – CCRC Outside Patio

Lunch

Distribute Evaluations

1:15 p.m.Pre-Selected Hands-on Experience – Modeling OR NMR:

Modeling Laboratory (Instructor: Rob Woods) (please install VMD & bring laptop)

  • Modeling glycomaterials with GLYCAM-Web
  • Visualization using VMD
  • Analysis with chimera

   OR

NMR Laboratory (Instructor: Jiri Vlach, Ambrish Kumar)

    • Sample preparation
    • Introduction to NMR instrument
  • 1D and 2D NMR Data acquisition and interoperation 
3:30 p.m.Reconvene

Complete, Submit Evaluations

Closing Comments (Rob Woods, Parastoo Azadi)

3:45 p.m.Adjourn, Afternoon Social Activity (TBD)