2023 GlycoMIP Summer School @ UGA Agenda

DAY ONE – Monday, Sept. 11: “Mass Spectrometry and Biolayer Interferometry of Glycans – Day 1”

8:15 AMArrival, Registration, Distribution of Parking Passes
8:30 AMWelcome, Introductions (Parastoo Azadi)
8:40 AMIntroduction to GlycoMIP (Parastoo Azadi)

  • What is GlycoMIP and how it can help your research 
  • Resources available at GlycoMIP for glycomaterial characterization 
  • How to interact with GlycoMIP and find what you need

GlycoBasics I/II (pre-work videos) Quiz 

9:05 AMSafety Overview (Mark Baine)
9:15 AMBreak
9:35 AMMass Spectrometry (MS) Lecture (Instructor: Parastoo Azadi)

  • Introduction to MS
  • Comparison of different MS instruments and capabilities 
  • Methods for glycomaterial MS analysis 
10:35 AMBreak
10:50 AMBio-Layer Interferometry (BLI) Lecture (Instructors: Rob Woods)

  • Introduction to BLI
  • Comparison of BLI with SPR and ITC
  • Assay Considerations 
11:50 AMBreak into Groups A & B
12:00 PMLunch; GlycoMIP Scholar Presentation – Structural Characterization of Glycomaterials using Mass Spectrometry (Ambrish Kumar)
1:15 PMGroup A:  MS Laboratory (Instructors: Stephanie Archer-Hartmann)

  • Spotting and running MALDI of glycomaterials
  • ESI-MS of glycomaterials (intact and digested)
  • Sequencing and linkage analysis by MS/MS (data interpretation) 

Group B:  BLI Laboratory (Instructors: Paige LaMore, Swanson Mahmoud)

  • Receptor Immobilization
  • Ligand binding and affinity quantification
  • Data Analysis
5:30 PMAdjourn, Group Dinner (Location TBD)

DAY TWO – Tuesday, Sept. 12: “Modelling; Mass Spectrometry and Biolayer Interferometry of Glycans – Day 2”

8:45 AMArrival, Parking Passes
9:00 AMModeling Lecture Part 1 (Instructor: Rob Woods)

  • Introduction to Molecular Modeling
  • Force fields
  • Energy minimization
  • Molecular dynamics simulation
10:30 AMBreak
10:45 AMModeling Lecture Part 2 (Instructor: Rob Woods)

  • Automated ligand docking
  • Positive controls
  • Literature examples
12:00 PMLunch; GlycoMIP Scholar Presentation – Biolayer Interferometry of Glycomaterials (Paige LaMore)
1:15 PMGroup A:  BLI Laboratory (Instructors: Paige LaMore, Sawsan Mahmoud)

    • Receptor Immobilization
    • Ligand binding and affinity quantification
  • Data Analysis 

Group B:  MS Laboratory (Instructors: Bhoj Kumar, Stephanie Archer-Hartmann)

  • Spotting and running MALDI of glycomaterials
  • ESI-MS of glycomaterials (intact and digested)
  • Sequencing and linkage analysis by MS/MS (data interpretation) 
5:30 PMAdjourn

DAY THREE – Wednesday, September 13: “Modeling and Nuclear Magnetic Resonance of Glycans”

8:45 AMArrival, Parking Passes
9:00 AMNMR (Nuclear Magnetic Resonance) Lecture (Instructor: Christian Heiss)

  • Introduction to NMR
  • Special considerations for NMR of glycomaterials
  • Interpretation of carbohydrate 1D and 2D -NMR spectra
12:00 PMGroup Photo – CCRC Outside Patio

Lunch

Distribute Evaluations

1:15 PMPre-Selected Hands-on Experience – Modeling OR NMR:

Modeling Laboratory (Instructor: Rob Woods) (please install VMD & bring laptop)

  • Modeling glycomaterials with GLYCAM-Web
  • Visualization using VMD
  • Analysis with chimera

   OR

NMR Laboratory (Instructor: Jiri Vlach, Christian Heiss)

    • Sample preparation
    • Introduction to NMR instrument
  • 1D and 2D NMR Data acquisition and interoperation 
3:30 PMReconvene

Complete, Submit Evaluations

Closing Comments (Rob Woods, Parastoo Azadi)

3:45 PMAdjourn, Afternoon Social Activity (TBD)