2023 GlycoMIP Summer School @ UGA Agenda

DAY ONE – Monday, September 11:Mass Spectrometry and Biolayer Interferometry of Glycans”

8:00 a.m.Arrival, Registration, Distribution of Parking Passes
8:15 a.m.Welcome, Introductions (Parastoo Azadi)
8:25 a.m.Introduction to GlycoMIP (Parastoo Azadi)

●      What is GlycoMIP and how it can help your research

●      Resources available at GlycoMIP for glycomaterial characterization

●      How to interact with GlycoMIP and find what you need

GlycoBasics I/II (pre-work videos) Quiz

8:50 a.m.Safety Overview (Mark Baine)

Break into Groups (A, B)

9:00 a.m.Break
9:10 a.m.

 

 

 

 

 

 

 

 

12:00 p.m.

Mass Spectrometry (MS) Lecture (Instructor: Parastoo Azadi)

●      Introduction to MS

●      Comparison of different MS instruments and capabilities

●      Methods for glycomaterial MS analysis

Bio-Layer Interferometry (BLI) Lecture (Instructor: Rob Woods/ Paige LaMore)

●      Introduction to BLI

●      Comparison of BLI with SPR and ITC

●      Assay Considerations

 

GlycoMIP Scholar Presentation (Instructor: Lauren Pepi)

12:50 p.m.Lunch
1:50 p.m.A:  MS Laboratory (Instructors: Lauren Pepi, Stephanie Archer-Hartmann)

●      Spotting and running MALDI of glycomaterials

●      ESI-MS of glycomaterials (intact and digested)

●      Sequencing and linkage analysis by MS/MS (data interpretation)

B:  BLI Laboratory (Instructors: Paige LaMore, Swanson Mahmoud)

●      Receptor Immobilization

●      Ligand binding and affinity quantification

●      Data Analysis

5:30 p.m.Adjourn, Group Dinner (Location TBD)

 

 

DAY TWO – Tuesday, September 12:Mass Spectrometry and Biolayer Interferometry of Glycans”

8:45 a.m.Arrival, Parking Passes
9:00 a.m.

 

 

 

12:00 p.m.

Modeling Lecture (Instructor: Rob Woods)

●      Introduction to Molecular Modeling and molecular dynamics simulation

●      Modeling the molecular scale

●      Sampling, relaxation, convergence, analysis and visualization

GlycoMIP Scholar Presentation (Instructor: Paige LaMore)

12:30 p.m.Lunch
1:30 p.m.A:  BLI Laboratory (Instructors: Paige LaMore)

B:  MS Laboratory (Instructor:  Lauren Pepi, Stephanie Archer-Hartmann)

5:30 p.m.Adjourn

 

 

DAY THREE – Wednesday, September 13:Modeling and NMR”

8:45 a.m.Arrival, Parking Passes
9:00 a.m.NMR (Nuclear Magnetic Resonance) Lecture (Instructor: Christian Heiss)

●      Introduction to NMR

●      Special considerations for NMR of glycomaterials

●      Interpretation of carbohydrate 1D and 2D -NMR spectra

12:00 a.m.Group Photo – CCRC Outside Patio

Lunch

1:15 p.m.Optional Hands-on Experience:

Modeling Laboratory (Instructor: Rob Woods) (please install VMD & bring laptop)

●      Modeling glycomaterials with GLYCAM-Web

●      Visualization using VMD

●      Analysis with chimera

OR

NMR Laboratory (Instructor: Jiri Vlach, Ambrish Kumar)

●      Sample preparation

●      Introduction to NMR instrument

●      1D and 2D NMR Data acquisition and interoperation

3:30 p.m.Complete, Submit Evaluations

Closing Comments (Rob Woods, Parastoo Azadi)

3:45 p.m.Adjourn, Afternoon Social Activity (TBD)