DAY ONE – Monday, September 11: “Mass Spectrometry and Biolayer Interferometry of Glycans”
| 8:00 a.m. | Arrival, Registration, Distribution of Parking Passes | ||
| 8:15 a.m. | Welcome, Introductions (Parastoo Azadi) | ||
| 8:25 a.m. | Introduction to GlycoMIP (Parastoo Azadi) ● What is GlycoMIP and how it can help your research ● Resources available at GlycoMIP for glycomaterial characterization ● How to interact with GlycoMIP and find what you need GlycoBasics I/II (pre-work videos) Quiz | ||
| 8:50 a.m. | Safety Overview (Mark Baine) Break into Groups (A, B) | ||
| 9:00 a.m. | Break | ||
| 9:10 a.m.
12:00 p.m. | Mass Spectrometry (MS) Lecture (Instructor: Parastoo Azadi) ● Introduction to MS ● Comparison of different MS instruments and capabilities ● Methods for glycomaterial MS analysis Bio-Layer Interferometry (BLI) Lecture (Instructor: Rob Woods/ Paige LaMore) ● Introduction to BLI ● Comparison of BLI with SPR and ITC ● Assay Considerations
GlycoMIP Scholar Presentation (Instructor: Lauren Pepi) | ||
| 12:50 p.m. | Lunch | ||
| 1:50 p.m. | A: MS Laboratory (Instructors: Lauren Pepi, Stephanie Archer-Hartmann) ● Spotting and running MALDI of glycomaterials ● ESI-MS of glycomaterials (intact and digested) ● Sequencing and linkage analysis by MS/MS (data interpretation) B: BLI Laboratory (Instructors: Paige LaMore, Swanson Mahmoud) ● Receptor Immobilization ● Ligand binding and affinity quantification ● Data Analysis | ||
| 5:30 p.m. | Adjourn, Group Dinner (Location TBD) |
DAY TWO – Tuesday, September 12: “Mass Spectrometry and Biolayer Interferometry of Glycans”
| 8:45 a.m. | Arrival, Parking Passes | ||
| 9:00 a.m.
12:00 p.m. | Modeling Lecture (Instructor: Rob Woods) ● Introduction to Molecular Modeling and molecular dynamics simulation ● Modeling the molecular scale ● Sampling, relaxation, convergence, analysis and visualization GlycoMIP Scholar Presentation (Instructor: Paige LaMore) | ||
| 12:30 p.m. | Lunch | ||
| 1:30 p.m. | A: BLI Laboratory (Instructors: Paige LaMore) B: MS Laboratory (Instructor: Lauren Pepi, Stephanie Archer-Hartmann) | ||
| 5:30 p.m. | Adjourn |
DAY THREE – Wednesday, September 13: “Modeling and NMR”
| 8:45 a.m. | Arrival, Parking Passes | ||
| 9:00 a.m. | NMR (Nuclear Magnetic Resonance) Lecture (Instructor: Christian Heiss) ● Introduction to NMR ● Special considerations for NMR of glycomaterials ● Interpretation of carbohydrate 1D and 2D -NMR spectra | ||
| 12:00 a.m. | Group Photo – CCRC Outside Patio Lunch | ||
| 1:15 p.m. | Optional Hands-on Experience: Modeling Laboratory (Instructor: Rob Woods) (please install VMD & bring laptop) ● Modeling glycomaterials with GLYCAM-Web ● Visualization using VMD ● Analysis with chimera OR NMR Laboratory (Instructor: Jiri Vlach, Ambrish Kumar) ● Sample preparation ● Introduction to NMR instrument ● 1D and 2D NMR Data acquisition and interoperation | ||
| 3:30 p.m. | Complete, Submit Evaluations Closing Comments (Rob Woods, Parastoo Azadi) | ||
| 3:45 p.m. | Adjourn, Afternoon Social Activity (TBD) |
