Summer School 2021 Agenda

A PDF version can be downloaded here.

NOTE:  All times are shown in Eastern Daylight Savings Time, EDT

DAY ONE – Monday, June 7:

10:45 a.m.Getting online and Check-In (Caudill)
11:00 a.m.Welcome and Introductions (Roman, Caudill)

GlycoBasics I
(Instructor:  Maren Roman)

  • Classes of glycomaterials
  • Natural occurrence and functions of glycomaterials
  • Glycomaterials-based technologies
12:00 p.m.Break
12:10 p.m.GlycoBasics II
(Instructor:  Kevin Edgar)

  • Building blocks of polysaccharides (PS)
  • Natural occurrence and functions of polysaccharides
  • Polysaccharide-based technologies
1:10 p.m.Extended Break
2:30 p.m.Synthesis: Fundamentals
(Instructor:  Peter Seeberger)

  • Protecting groups
  • Formation of glycosidic linkage
  • Oligosaccharide synthesis strategies
3:30 p.m.Break
3:40 p.m.Synthesis:  Application
(Instructor:  Peter Seeberger)

  • History of automated glycan assembly (AGA)
  • Opportunities for AGA in glycomaterials R&D
  • Current limitations of AGA
4:40 p.m.Adjourn


DAY TWO – Tuesday, June 8:

10:45 a.m.Getting online and Check-In (Caudill)
11:00 a.m.Analytical:  Mass Spectrometry – Fundamentals
(Instructor: Rich Helm)

  • Mass spectrometry basics
  • Mass spectrometry and LCMS hardware
  • Basic experiments using MALDI and LCMS technologies
12:00 p.m.Break
12:10 p.m.Analytical:  Mass Spectrometry – Application
(Instructor:  Parastoo Azadi)

  • Sample type and preparation/isolation/purification
  • Derivatization of glycans for glycan mass spectrometry
  • Fragmentation of glycans for sequencing and linkage formation
1:10 p.m.Extended Break
2:30 p.m.Analytical:  Nuclear Magnetic Resonance (NMR) – Fundamentals
(Instructor:  John Glushka)

  • Experimental concepts, chemical shifts, J-coupling, relaxation
  • Types of NMR spectra (1D 1H, 13C; 2D 1H, 1H-13C correlation)
  • Key experimental parameters for carbohydrate NMR data
3:30 p.m.Break
3:40 p.m.Analytical:  NMR – Application
(Instructor:  Christian Heiss)

  • Utility of 2D NMR experiments
  • Special considerations for the NMR of carbohydrates
  • Interpretation of carbohydrate spectra
4:40 p.m.Adjourn

 

DAY THREE – Wednesday, June 9:

10:45 a.m.Getting online and Check-In (Caudill)
11:00 a.m.Analytical:  Carbohydrate – Protein Binding Assays – Fundamentals
(Instructor:  Alan Esker)

  • General principles
  • Methods – SPR, BLI, MST and ITC
12:00 p.m.Break
12:10 p.m.Analytical:  Carbohydrate – Protein Binding Assays – Application
(Instructor:  Rob Woods)

  • Binding assay (Kd, IC50, etc.)
  • Example applications of BLI
  • Limitations of BLI (and other methods)
1:10 p.m.Extended Break
 

2:30 p.m.

 
Analytical:  Rheometry – Fundamentals
(Instructor:  Blake Johnson)

  • Rheometry basics
  • Rheometry hardware
  • Basic rheometry applications in biomaterials analysis
3:30 p.m.Break
3:40 p.m.Analytical:  Rheometry – Application
(Instructor:  Ross Clark)

  • Selecting an appropriate methodology for the test
  • Some common examples (inkjet printing, cosmetics, beverage mouthfeel, emulsion stability, fire retardant application)
  • Common errors made in test selection and application
4:40 p.m.Adjourn

 

DAY FOUR – Thursday, June 10:

10:45 a.m.Getting online and Check-In (Caudill)
11:00 a.m.Modeling:  Computational Methods for Glycomaterials – Fundamentals
(Instructor:  Valerie Welborn)

  • Overview of computational methods
  • Applications of quantum mechanics-based methods
  • Applications of molecular mechanics-based methods
12:00 p.m.Break
12:10 p.m.Modeling:  Computational Methods for Glycomaterials – Application
(Instructor:  Rob Woods)

  • Docking carbohydrates to proteins
  • Glycoscience applications of MD simulations
  • Limitations of MD simulations
  • Capabilities of Glycam.org
1:10 p.m.Extended Break
2:30 p.m.Analytical:  Chiroptical Spectroscopy – Fundamentals
(Instructor:  Daniel Crawford)

  • Chirality and optical properties
  • Chirality and vibrational spectroscopy
  • Challenges of chiroptical spectroscopy for carbohydrates
3:30 p.m.Break
3:40 p.m.Analytical:  Chiroptical Spectroscopy – Application
(Instructor:  Prasad Polavarapu)

  • Raman and Infrared spectroscopy fundamentals
  • Vibrational circular dichroism of carbohydrates
  • Raman optical activity of carbohydrates
4:40 p.m.Adjourn

 

DAY FIVE – Friday, June 11:

10:45 a.m.Getting online and Check-In (Caudill)
11:00 a.m.Interactive Panel Discussion #1:  GlycoBasics and Synthesis
Panelists:

  • Maren Roman
  • Rich Helm
  • Kevin Edgar
Break
11:45 a.m.Interactive Panel Discussion #2:  Modeling
Panelists:

  • Rob Woods
  • Valerie Welborn
  • Sanket Deshmukh
Break
12:30 p.m.Interactive Panel Discussion #3:  Analytical
Panelists:

  • Parastoo Azadi
  • Alan Esker
  • Christian Heiss
1:15 p.m.Closing Remarks
1:30 p.m.Adjourn