2024 GlycoMIP Summer School @ UGA Agenda

DAY ONE

Monday, Sept. 23: “Mass Spectrometry and Biolayer Interferometry of Glycans”

Day One Schedule
Time	Session	Location
8:15 AM	Arrival, Registration, Distribution of Parking Passes
8:30 AM	Welcome, Introductions (Parastoo Azadi)	Rm 1036
8:40 AM	Introduction to GlycoMIP (Parastoo Azadi) What is GlycoMIP and how it can help your research Resources available at GlycoMIP for glycomaterial characterization How to interact with GlycoMIP and find what you need	Rm 1036
9:05 AM	Safety Overview (Mark Baine)	Rm 1036
9:15 AM	Break
9:35 AM	Mass Spectrometry (MS) Lecture (Instructor: Parastoo Azadi) Introduction to MS Comparison of different MS instruments and capabilities Methods for glycomaterial MS analysis Review of reading materials	Rm 1036
10:35 AM	Break
10:50 AM	Bio-Layer Interferometry (BLI) Lecture (Instructor: Sawsan Mahmoud) Introduction to BLI Comparison of BLI with SPR and ITC Inhibition (IC50) Assay Considerations Review of reading materials	NMR Overlook
12:00 PM	Lunch; GlycoMIP Scholar Presentation (20 min) – “Biolayer Interferometry of Glycomaterials” (Sawsan Mahmoud)	CCRC Breakroom
1:10 PM	Split into Groups A & B
1:15 – 5:30 PM	Group A: MS Laboratory (Instructors: Stephanie Archer-Hartmann & Parastoo Azadi, Bhoj Kumar) Spotting and running MALDI of glycomaterials ESI-MS of glycomaterials (intact and digested) Sequencing and linkage analysis by MS/MS (data interpretation) Group B: BLI Laboratory (Instructor: Sawsan Mahmoud) Receptor Immobilization Ligand binding and affinity quantification Data Analysis	Rm 1036 / Rm 2069
5:30 – 6:30 PM	Adjourn Group Dinner: The Rook & Pawn 294 W Washington St, Athens, GA 30601

DAY TWO

Tuesday, Sept. 24: “Modeling; Mass Spectrometry and Biolayer Interferometry of Glycans”

Day Two Schedule
Time	Session	Location
8:45 AM	Arrival, Distribution of Parking Passes
9:00 AM	Modeling Lecture Part 1 (Instructor: Lachele Foley) Please pre-install VMD and* ChimeraX software and bring a laptop Introduction to Molecular Modeling Force fields Energy minimization Automated ligand docking	Rm 1036
10:30 AM	Break
10:45 AM	Modeling Lecture Part 2 (Instructor: Lachele Foley) Molecular dynamics simulations Predicting oligosaccharide 3D structures Online modeling tools: GLYCAM-WEB	Rm 1036
12:00 PM	Lunch: GlycoMIP Scholar Presentation (20 min) – “Structural Characterization of Glycomaterials by MALDI-MS Imagining Technologies (Sergei Shalygin)	CCRC Breakroom
1:15 – 5:30 PM	Group A: BLI Laboratory (Instructor: Sawsan Mahmoud) Receptor Immobilization Ligand binding and affinity quantification Data Analysis Group B: MS Laboratory (Instructors: Stephanie Archer-Hartmann & Bhoj Kumar) Spotting and running MALDI of glycomaterials ESI-MS of glycomaterials (intact and digested) Sequencing and linkage analysis by MS/MS (data interpretation)	Rm 2069 / Rm 1025
5:30 PM	Adjourn, Dinner on your own

DAY THREE

Wednesday, September 25: “Nuclear Magnetic Resonance of Glycans”

Day Three Schedule
Time	Session	Location
8:45 AM	Arrival, Distribution of Parking Passes
9:00 AM	NMR (Nuclear Magnetic Resonance) Lecture (Instructor: Christian Heiss) Introduction to NMR (John Glushka: 5 min & 1-2 slides) Special considerations for NMR of glycomaterials Interpretation of carbohydrate 1D and 2D -NMR spectra	Rm 1036
10:15 AM	Break
10:45 AM	NMR Lecture (Continued)
12:00 PM	Group Photo Lunch Distribute Evaluations	CCRC Patio & Breakroom
1:15 – 5:00 PM	NMR Laboratory (Instructors: Jiri Vlach & Ambrish Kumar) Please pre-install Mnova (trial version) & bring laptop)* Sample preparation Introduction to NMR instrument 1D and 2D NMR data acquisition and interpretation Modeling Laboratory (Instructor: Lachele Foley). Molecular dynamics simulations hands-on Predicting oligosaccharide 3D structures Online modeling tools: GLYCAM-WEB	Rm 1036 / Rm 1203, CMM
5:00 PM	Reconvene Collect/Submit Course Evaluations Closing Comments (Parastoo Azadi)	Rm 1036
5:30 PM	Adjourn